Real-time analysis at 600 samples per hour

Measures: total bacteria count and somatic cell count

The BactoCount IBCm is a semi-automated instrument that uses flow cytometry for the rapid enumeration of individual bacteria and somatic cells in milk. The rapid test for bacteria makes it an ideal solution for any processing plant or test laboratory involved in quality assessment on milk.

The IBCm is a general flow cytometer and its applications have been expanded to include the counting of somatic cells in raw milk. This gives a laboratory a convenient 2-for-1 solution when making laboratory investments.

  • Analyzing time for bacterial count is less than one minute and sample preparation is completed in less than 10 minutes
  • Analyzing time for somatic cell count is less than one minute, no sample preparation needed
  • Use of a standard computer offers flexible data output options
  • Easy operator-assisted sample preparation
  • Low-maintenance design

Recognized by both NCIMS and MicroVal organizations. The BactoCount IBCm is based on the BactoCount IBC 50-150 models which have been approved as an alternative method to the IDF 100B:1991 reference method for the rapid and accurate determination of the hygienic quality of cow, sheep and buffalo raw milk for payment purposes. The IBC meets AOAC 986.33. Validated by MicroVal to be at least equivalent to the EN-ISO 4833-1:2013 and 4833-2:2013 reference methods for the enumeration of microorganisms in raw cow milk (Certificate n° 2013LR44)

Technical overview and principle of operation

The practical application varies slightly depending on whether it is somatic or bacterial cells that are counted but the general principle is the same: the cells are stained with a fluorescent dye that inserts into the intracellular DNA followed by detection and enumeration by a flow cytometry based cell counter.

The IBCm consists of three modules:

1. The counter

The counting assembly includes a powerful solid state laser, a flow cell, a set of optics, a narrow band filter, and a highly sensitive photo multiplier. The cells are aligned in the flow cytometer and exposed to an intense laser beam to excite the fluorescent marker intercalated into the intracellular DNA. The fluorescence pulses are collected with the optics, filtered with a narrow band filter, and detected with the photo multiplier. The pulses are sorted based on duration and intensity and then translated into individual bacteria counts (IBC’s) or somatic cell counts (SCC’s) depending on the chosen application. Using parallel results of IBC’s and traditional plate counts the instrument can be calibrated to output results in CFU/mL. Similarly, commercially available standards/reference samples with predetermined numbers of somatic cells can be paired with counts obtained with the IBCm to obtain the numbers in DMSCC/mL. (direct microscopic somatic cell count). The counting assembly is compact, enclosed and regulated at 30°C (86°F) to provide a highly stable environment.

2.  The incubator

The main element of the incubator is a heated plate where six stainless steel vials can be placed side-by-side. The incubation plate is temperature regulated at 50°C (122°F) to optimize the process. The incubator also features a funnel-shaped slot to conveniently store the sonicator in for quick and easy access.

3.   The computers

The IBCm is equipped with a powerful internal industrial computer. The internal computer is used to operate the instrument and send all the data and diagnostics to the external computer. The external computer is a standard personal PC or workstation running a version of Microsoft Windows (currently the IBCm software has been tested on the following platforms: Windows 95, Windows 98, Windows 2000, and Windows XP).

Analysis performanceThe graphs below display typical peak, width and scatter distributions obtained with the BactoCount method of the predominant bacteria species used for the assessment of the hygienic quality of raw milk:

Our history and future

Founded in 1982, Bentley Instruments, Inc. specializes in the development, manufacturing and worldwide distribution of analytical instruments for milk and milk products. Since the beginning, we have focused on developing solutions that set the standard for reliability, precision and ease of use.

Bentley Instruments has always been on the leading edge. Our engineers pioneered the computer control philosophy for dairy product analysis equipment. We combined standard computer electronics with robust instrument design to create laboratory instruments that both improved and simplified the testing process. Additionally, Bentley Instruments was the first to offer flow cytometry for routine milk analysis.

Our innovations have helped us secure a strong presence in the specialized market of milk and dairy product analysis. We now export more than 70% of our products to areas outside the U.S., and our market share is still growing.

In the years to come, we will continue to develop cutting-edge solutions for the dairy industry. Our commitment to innovation is constant.

Поверка

осуществляется по документу МП 12-241(243)-2018 «ГСИ. Анализаторы молока «Bentley Instruments». Методика поверки», утверждённому ФГУП «УНИИМ» 03 мая 2018 г.

Основные средства поверки:

—    Г осударственный первичный эталон единиц массовой доли, массовой (молярной) концентрации воды в твердых и жидких веществах и материалах ГЭТ 173-2017;

—    Государственный вторичный эталон единиц массовой доли и массовой (молярной) концентрации компонентов в твердых и жидких веществах и материалах на основе объемного титриметрического метода анализа ГВЭТ 176-1-2010, № 2.1.ZZC.0148.2014;

—    Государственный вторичный эталон единиц массовой доли и массовой (молярной) концентрации органических компонентов в жидких и твердых веществах и материалах на основе газовой и жидкостной хроматографии ГВЭТ 208-1-2016, № 2.1.ZZC.0259.2017.

Допускается применение аналогичных средств поверки, обеспечивающих определение метрологических характеристик поверяемых СИ с требуемой точностью.

Знак поверки наносится на свидетельство о поверке.

Your needs come first

Customer satisfaction is our first priority. To ensure that our solutions meet your needs, we focus on five key points:

Quality: Our instruments are engineered and constructed for years of reliable use in real-world environments.Simplicity: Our instruments are easy to operate, with sample input and intuitive touch-screen interfaces.Value: Today more than ever, cost is a serious consideration. Our tools deliver exceptional value.Service: Our instruments are easy to service and troubleshoot. Support: We understand the critical role our instruments play at your organization, and how much you depend on their reliable operation. If a challenge arises, we do what it takes to solve the issue quickly and get your process back on track. 

Measures: Somatic cell counts

The Somacount FCM delivers the ultimate efficiency and accuracy in early mastitis detection. This instrument provides a rapid and accurate count for the SCC (somatic cell count) in dairy milk.

This instrument represents the latest in state-of- the-art technology for automated milk analysis at up to 600 samples per hour. Engineered in accordance with Bentley Instruments’ rigorous design principles, it provides precise and accurate measurements. The Somacount flow cytometer, provides an accurate count of the somatic cells in a raw milk sample (cow, sheep, goat, camel, buffalo etc.). After first being stirred, the milk is drawn from a sample vial and delivered to the measurement module. The sampling, sequencing and identification of the sample vials are functions performed by the Autosampler.

Fully integrated software controlsWith this instrument, control of the Somacount FCM module is integrated into a single Windows-based software program. The simple and intuitive graphical user interface is designed for easy operator use. The software’s sophisticated data export and reporting functions and network access capability allow laboratory supervisors to monitor instrument status and analyze results remotely.

Technology summary

The Somacount FCM is the next innovation in the evolution of our world-renowned instruments for somatic cell counting. Based on a highly stable solid- state laser induced fluorescence, this module is capable of delivering an accurate and timely warning for mastitis onset.

Testing standardsThe Bentley FCM exceeds the IDF 148A standard and ICAR requirements for somatic cell counting, employing AOAC approved methodology.

Technical overview of Somacount FCM moduleThe Somacount FCM is based on proven flow cytometry methodology for somatic cell counting. This system uses a proprietary process based on the principle of laser-based flow cytometry to determine the somatic cell count in raw milk. Flow cytometry is an extremely powerful and versatile technique and is the method of choice in the medical field for detecting, analyzing and sorting cells. The Somacount FCM is the result of 20+ years of experience and research in flow cytometry; Bentley Instruments pioneered the use of cytometry in the central laboratories with the introduction of the Somacount 300 developed in 1991.

Remote diagnostic capabilitiesThe web-enabled diagnostic features of the FCM series allow Bentley Instruments to access your machine from any location. This enables your laboratory personnel and Bentley Instruments to collaborate and analyze all system functionalities, no matter where the instrument is located.

Service Dedicated to Your Success

As a company completely focused on the needs of the dairy industry, we understand the challenges of today’s laboratories and large dairies. Bentley Instruments has a global reputation for delivering the highest level of customer service and support, focused on enhancing the operational efficiency that is absolutely critical to all of our customers.

For Bentley Instruments, service includes instrument reliability, support and local service:

  • Instrument Reliability: Bentley Instruments is focused on engineering instruments that are easy to operate, and highly reliable. Many of our instruments have been at work for over 20 years, and all are built with robust construction techniques that help them prove their value for years to come.
  • Technical Support: At Bentley Instruments, technical inquiries from our customers are quickly handled by our engineers and product specialists. Our mission is to keep you running 24/7 and help you utilize your investment and to maximize the efficiency of your laboratory. In short we will do whatever it takes to quickly solve any technical inquiry.
  • Local Representatives: With representatives all over the world, our local support staff is there to provide after the sale service you can rely on, on-site or remotely as the situation demands.

Программное обеспечение

Анализаторы имеют программное обеспечение (далее ПО), которое обеспечивает математическую обработку результатов измерения, накопление их в базе данных, а также диагностику и управление. ПО делится на метрологически значимую часть и сервисную часть программы. Метрологически значимая часть ПО реализует обработку результатов измерений содержания компонентов, запись полученных результатов в память анализатора и представление измерительной информации на мониторе компьютера. Защита ПО от непреднамеренных и преднамеренных изменений соответствует уровню «средний» по Р 50.2.077-2014. Идентификационные данные ПО приведены в таблице 1. Влияние ПО на метрологические характеристики учтено при их нормировании.

Таблица 1 — Идентификационные данные ПО

Идентификационные данные (признаки)

Значение

Идентификационное наименование ПО

V 2.17.5876

Номер версии (идентификационный номер) ПО

не ниже 38896

Цифровой идентификатор ПО

Measures: somatic cells, fat, protein, lactose and solids

The Bentley DairySpec & SomaCount Combi simultaneously measures somatic cells and components, helping dairy laboratories to greatly enhance their operation’s efficiency. This system combines the analytically power of the DairySpec FT and the Somacount FC, two of Bentley Instruments most proven, reliable and easy to use systems.  A single auto-sampler delivers samples to both systems, speeding up the analysis process. Optional bar code readers can also be used to monitor and track samples.

DairySpec FT (component analysis module) features

The DairySpec FT is a compact, highly accurate instrument for the analysis of milk and milk products.

  • Analyzes dairy components including fat, protein, lactose and solids
  • Up to 150 samples per hour
  • Handles products of high viscosity
  • Flexible data output options
  • Optional Autosampler for fully automatic operation
  • Available with Fat “A” and Fat “B”
  • Optional freezing point depression (FPD)
  • Low maintenance design

Accurate, compact, fast and reliable, the Bentley 150 is ideal for laboratories with small to medium testing requirements.

Technical overview & principle of operation The DairySpec FT consists of a proprietary optical infrared analysis system and an integrated high speed computer. To measure specific components within milk products, the instrument measures the energy absorption at specific wavelengths in the mid-infrared region.

  • Operators use computer controls to vary the pump and analysis speed, directing the system to operate at a slower speed when processing products of high viscosity, such as cream.
  • Programmable screen layout allows the operator to choose up to eight windows to display component values, both measured and calculated.
  • The instrument is capable of handling multiple calibrations as needed to optimize accuracy in testing different components.
  • Operator friendly software with graphic user interface (GUI) helps make the system easy to operate.
  • The flexible design of the instrument allows it to be used as a product analyzer capable of testing a wide variety of fluid dairy products.
  • Instrument durability allows for use in high volume testing environments.
  • State-of-the-art design utilizes few moving parts, making the system very easy to maintain.
  • Small, compact size and integrated computer reduces the amount of bench space needed, allowing the system to be used in the smallest of laboratories.

SomaCount FC(somatic cell counting module) features

The SomaCount FCdelivers a rapid and accurate measurement somatic cell counts (SCC) for early mastitis detection in dairy milk. This proven system is designed for reliability, ease-of-use and precision measurements.

This instrument is ideal for small to mid-size laboratories that need an easy-to-maintain somatic cell counting system.

  • Capable of analyzing over 150 samples per hour
  • Built-in computer offers flexible data output options
  • Optional Autosampler for fully automated analysis
  • Low maintenance design

Technical overview & principle of operationThe SomaCount 150 uses a proprietary process based on the principle of laser-based flow cytometry to determine the somatic cell counts in a milk sample.

  • A milk sample is taken automatically and mixed with a fluorescent dye solution.
  • This dye disperses the fat globules and stains the DNA in the somatic cells.
  • An aliquot part of the stained suspension is injected into a laminar stream of carrier fluid.
  • The somatic cells are separated by the stream and exposed to a laser beam.
  • As the stained cells pass through the excitation source (laser beam), they begin to fluoresce creating a light pulse.
  • Through a series of lenses, the fluorescent pulses are focused onto a photo-multiplier tube, where they are converted into electrical pulses.
  • The pulses are sorted and stored by size using a micro-controller. By using a process known as pulse height analysis, the pulses are sorted, counted and translated into a somatic cell count.
  • The software allows for a wide variety of data output options, including diskette, serial, parallel and network connections which can be easily formatted to fit into the user’s existing system.
  • Operator friendly software with graphic user interface (GUI) helps make the system easy to operate.
  • The Somacount FCmeets the requirements of IDF standards for somatic cell counting. It is an AOAC approved methodology.

Measures: fat, protein, lactose and more

The Bentley FTS represents the latest in state-of-the-art technology for automated milk analysis at 600 samples per hour. Engineered in accordance with Bentley Instruments’ rigorous design principles, it provides precise and accurate measurements. The Bentley FTS uses the Fourier Transform Spectrometer (FTIR) to measure the milk composition, including fat, protein and lactose. After first being stirred, the milk is drawn from a sample vial and delivered to the measurement module. The sampling, sequencing and identification of the sample vials are functions performed by the Autosampler.

Fully integrated software controlsWith this advanced instrument, control of the FTS module is integrated into a single Windows-based software program. The simple and intuitive graphical user interface is designed for easy operator use. The software’s sophisticated data export/reporting functions and network access capability allow laboratory supervisors to monitor instrument status and analyze results remotely.

Technology summaryThe Bentley FTS component analyzer uses an industrial Fourier Transform Spectrometer (FTIR) that captures the complete infrared absorption spectrum of the milk sample for component analysis. Collecting the complete spectrum allows the calibration to be based on all spectral characteristics of each particular component. The infrared spectrum may be permanently stored so that other calibrations may be retroactively applied to your data at any time in the future.

Testing standardsThe Bentley FTS exceeds the IDF 141C:2000 and ICAR requirements for component measurement employing AOAC approved methodology.

Technical overview of the Bentley FTS moduleThe Bentley FTS contains a Fourier Transform Spectrometer (FTIR). The optical deck of the FTS is housed in a hermetically-sealed enclosure, ensuring enhanced temperature stability and environmental robustness. The air in the sealed deck is pumped out, leaving the deck under vacuum. The combination of the hermetic seal and removing the humid air greatly reduces the humidity load on the enclosed desiccant, virtually eliminating the preventative maintenance task of frequent desiccant regeneration.

Remote diagnostic capabilitiesThe web-enabled diagnostic features of the Bentley FTS model allow Bentley Instruments to access your machine from any location. This enables your laboratory personnel and Bentley Instruments to collaborate and analyze all system functionality, no matter where the instrument is located.

Other Bentley FTS features
(click image to view)

вариабельНый тахиметр — использоваНие

Данные о расстоянии всегда помещайте напротив отметки, служащей в качестве основы для расчетов:

  • на SECONDS (секунды), если интервал измеряется в секундах;
  • на MINUTES (минуты), если интервал измеряется в минутах;
  • на HOURS (часы), если интервал измеряется в часах.

примечание: Показания времени, полученные при измерении с помощью хронографа, должны быть в случае необходимости переведены в секунды, минуты или часы, чтобы они соответствовали одной из трех отметок на неподвижной шкале. Например, 1 минута 20 секунд = 80 секунд; 1 час 10 минут = 70 минут и т.д.

пример

при условии, что автомобиль преодолеет 1,5 км (или 1,5 мили) за 45 секунд, какова его средняя скорость?

Salon Forum Labo Paris 2019

July 2nd, 2018

Bentley Instruments is waiting for you at the Forum Labo Paris international exhibition dedicated to analysis, biotechnology, control and research for public and private laboratories. This fair brings together 400 companies for a total of 8600 visitors and 150 innovations.

As an expert in the field of milk analysis, Bentley Instruments will be delighted to introduce the DairySpec FT, a precise and reliable milk analysis tool incorporating the new technologies of the NexGen series. With its experience and its links with the laboratories, we never stop improving processes. This allows us for example to obtain bacteriological parameters in less than 10 minutes, and chemical in less than 6 minutes.

Our team will be at your disposal on site to welcome and explain how Bentley Instruments offers real efficiency in your professional activity!

The show will take place from 26 to 28 March 2019, Porte de Versailles, Paris (more information www.forumlabo.com).

You are welcome at Forum Labo!

Measures: somatic cell counts, fat, protein, lactose and more

The Bentley FTS/FCM series represents the latest in state-of- the-art technology for automated milk analysis at up to 600 samples per hour. Engineered in accordance with Bentley Instruments’ rigorous design principles, the FTS/FCM provides precise and accurate measurements. The FTS/FCM comprises two separate modules—the Bentley Flow Cytometer (FCM), and the Bentley Fourier Transform Spectrometer (FTS). The FCM measures the somatic cell count of the milk samples while the FTS simultaneously measures the milk composition, including fat, protein and lactose. After first being stirred, the milk is drawn from a sample vial and delivered to both measurement modules. The sampling, sequencing and identification of the sample vials are functions performed by the Autosampler.

Fully integrated software controlsWith this series, control of both the FCM and FTS modules is integrated into a single Windows-based software program. The simple and intuitive graphical user interface is designed for easy operator use. The software’s sophisticated data export/reporting functions and network access capability allow laboratory supervisors to monitor instrument status and analyze results remotely.

Technology summaryThe FTS component analyzer uses an industrial Fourier Transform Spectrometer (or FTIR) that captures the complete infrared absorption spectrum of the milk sample for component analysis. Collecting the complete spectrum allows the calibration to be based on all spectral characteristics of each particular component. The infrared spectrum is permanently stored so that at any time in the future other calibrations may be retroactively applied to your data.

The FCM is the next innovation in the evolution of our world-renowned instruments for somatic cell counting. Based on a highly stable solid-state laser induced fluorescence, this module is capable of delivering an accurate and timely warning for mastitis onset.

Testing standardsThis FTS/FCM series exceeds the IDF 148A standard and ICAR requirements for somatic cell counting and IDF 141C:2000 and ICAR requirements for component measurement employing AOAC approved methodology.

Technical overview of FTS moduleThis module uses a Fourier Transform Spectrometer (or FTIR) to capture the complete infrared absorption spectrum of the milk sample for component analysis. Collecting the complete spectrum allows the calibration to be based on multiple spectral characteristics of each particular component. The optical deck of the FTS is housed in a hermetically sealed enclosure, ensuring enhanced temperature stability and environmental robustness. The air in the sealed deck is pumped out, leaving the deck under vacuum. The combination of the hermetic seal and removing the humid air greatly reduces the humidity load on the enclosed desiccant, virtually eliminating the preventative maintenance task of frequent desiccant regeneration.

Technical overview of FCM moduleThe FCM is based on proven flow cytometry methodology for somatic cell counting. This system uses a proprietary process based on the principle of laser-based flow cytometry to determine the somatic cell count in raw milk. Flow cytometry is an extremely powerful and versatile technique and is the method of choice in the medical field for detecting, analyzing and sorting cells. The Somacount FCM is the result of 15 years of experience and research in flow cytometry; the first instrument was developed in 1991.

Remote diagnostic capabilitiesThe web-enabled diagnostic features of the FTS/FCM series allow Bentley Instruments to access your machine from any location. This enables your laboratory personnel and Bentley Instruments to collaborate and analyze all system functionalities, no matter where the instrument is located.

Other features
(click image to view)

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